验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, SMMC-7721 cells, COLO 320 cells, Caco-2 cells, SW 1990 cells, BxPC-3 cells, PC-3 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
66381-1-Ig targets SLCO1B3/OATP1B3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | SLCO1B3/OATP1B3 fusion protein Ag19033 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier organic anion transporter family, member 1B3 |
| 别名 | LST 2, LST 3TM13, LST2, LST3, OATP 8, OATP1B3, OATP8, Organic anion transporter 8, SLC21A8, SLCO1B3 |
| 计算分子量 | 702 aa, 77 kDa |
| 观测分子量 | 68-77 kDa |
| GenBank蛋白编号 | BC105597 |
| 基因名称 | SLCO1B3 |
| Gene ID (NCBI) | 28234 |
| RRID | AB_2881757 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9NPD5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLCO1B3, also known as OATP8 or OATP1B3, is a member of Organic Anion Transporting Polypeptides (OATPs) which are sodium-independent organic anion transporters mediating the uptake of a wide range of structurally diverse endogenous and exogenous compounds including bile acids, hormone conjugates, peptides, toxins, as well as a multitude of therapeutic drugs. SLCO1B3 is specifically expressed in liver under normal condition, while its expression has also been observed in cancer tissues like colon, prostate and pancreas. It corresponds to Oatp1b2 in mice, with the gene symbol Slco1b2. The mouse genome encodes only a single transporter gene in the OATP1B subfamily, because SLCO1B1 and SLCO1B3 arose in primates by gene duplication after divergence from rodents. The molecular mass of SLCO1B3 is highly dependent on the glycosylation modification (deglycosylated form of 65 kDa, fully-glycosylated form of 120 kDa).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SLCO1B3/OATP1B3 antibody 66381-1-Ig | Download protocol |
| IHC protocol for SLCO1B3/OATP1B3 antibody 66381-1-Ig | Download protocol |
| IF protocol for SLCO1B3/OATP1B3 antibody 66381-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) SLCO1B3 promotes colorectal cancer tumorigenesis and metastasis through STAT3. | ||
Nat Commun Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity | ||
Bull Exp Biol Med The Effect of Original Russian Neurotropic Drugs on Organic Anion Transporting Polypeptides OATP1B1 and OATP1B3 | ||
Aging (Albany NY) Comprehensive analysis of scRNA-seq and bulk RNA-seq reveal the characteristics of disulfidptosis and a prognostic signature in BLCA | ||
Bull Exp Biol Med Regulation of the Organic Anion Transporting Polypeptide 1B3 (OATP1B3) by Sex Hormones | ||
Sci Rep OATP1B3 (699G>A) and CYP2C9*2, *3 significantly influenced the transport and metabolism of glibenclamide and glipizide. |