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SLCO1B3/OATP1B3 Monoclonal antibody, PBS Only

SLCO1B3/OATP1B3 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D9A4

Cat No : 66381-1-PBS

Print datasheet

Synonyms

LST 2, LST 3TM13, LST2, LST3, OATP 8, OATP1B3, OATP8, Organic anion transporter 8, SLC21A8, SLCO1B3

验证数据展示

  • IF Staining of HepG2 using 66381-1-Ig (same clone as 66381-1-PBS)

    IF Staining of HepG2 using 66381-1-Ig (same clone as 66381-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLCO1B3/OATP1B3 antibody (66381-1-Ig, Clone: 1D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66381-1-Ig (same clone as 66381-1-PBS)

    WB analysis of HepG2 using 66381-1-Ig (same clone as 66381-1-PBS)

    Western blot analysis of SLCO1B3/OATP1B3 in various cell lines using Proteintech antibody 66381-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66381-1-Ig (same clone as 66381-1-PBS)

    IHC staining of human liver cancer using 66381-1-Ig (same clone as 66381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3/OATP1B3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66381-1-Ig (same clone as 66381-1-PBS)

    IHC staining of human liver cancer using 66381-1-Ig (same clone as 66381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66381-1-PBS in a different storage buffer formulation.

产品信息

66381-1-PBS targets SLCO1B3/OATP1B3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen SLCO1B3/OATP1B3 fusion protein Ag19033 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name solute carrier organic anion transporter family, member 1B3
Synonyms LST 2, LST 3TM13, LST2, LST3, OATP 8, OATP1B3, OATP8, Organic anion transporter 8, SLC21A8, SLCO1B3
Calculated Molecular Weight 702 aa, 77 kDa
Observed Molecular Weight 68-77 kDa
GenBank Accession NumberBC105597
Gene Symbol SLCO1B3
Gene ID (NCBI) 28234
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NPD5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLCO1B3, also known as OATP8 or OATP1B3, is a member of Organic Anion Transporting Polypeptides (OATPs) which are sodium-independent organic anion transporters mediating the uptake of a wide range of structurally diverse endogenous and exogenous compounds including bile acids, hormone conjugates, peptides, toxins, as well as a multitude of therapeutic drugs. SLCO1B3 is specifically expressed in liver under normal condition, while its expression has also been observed in cancer tissues like colon, prostate and pancreas. It corresponds to Oatp1b2 in mice, with the gene symbol Slco1b2. The mouse genome encodes only a single transporter gene in the OATP1B subfamily, because SLCO1B1 and SLCO1B3 arose in primates by gene duplication after divergence from rodents. The molecular mass of SLCO1B3 is highly dependent on the glycosylation modification (deglycosylated form of 65 kDa, fully-glycosylated form of 120 kDa).