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验证数据展示

  • WB analysis of human cerebellum using 66439-1-Ig

    WB analysis of human cerebellum using 66439-1-Ig

    human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 66439-1-Ig

    IHC staining of human brain using 66439-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 66439-1-Ig

    IHC staining of human brain using 66439-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 66439-1-Ig

    IF Staining of mouse brain using 66439-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse brain using 66439-1-Ig

    IF Staining of mouse brain using 66439-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

KChIP1 Monoclonal antibody

KChIP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66439-1-Ig

产品说明书

CloneNo. 3D6C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman cerebellum tissue, fetal human brain tissue, human spleen tissue
Positive IHC detected inhuman brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66439-1-Ig targets KChIP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 KChIP1 fusion protein Ag5494 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Kv channel interacting protein 1
别名 KCHIP1, KCNIP1, VABP, Vesicle APC binding protein
计算分子量 227 aa, 27 kDa
观测分子量 25 kDa
GenBank蛋白编号BC050375
基因名称 KCNIP1
Gene ID (NCBI) 30820
RRIDAB_2881809
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NZI2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.

实验方案

Product Specific Protocols
WB protocol for KChIP1 antibody 66439-1-IgDownload protocol
IHC protocol for KChIP1 antibody 66439-1-IgDownload protocol
IF protocol for KChIP1 antibody 66439-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols