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验证数据展示

  • WB analysis of Raji using 66449-1-Ig

    WB analysis of Raji using 66449-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of human tonsillitis using 66449-1-Ig

    IF Staining of human tonsillitis using 66449-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human tonsillitis using 66449-1-Ig

    IF Staining of human tonsillitis using 66449-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human tonsillitis using 66449-1-Ig

    IHC staining of human tonsillitis using 66449-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66449-1-Ig

    IHC staining of human tonsillitis using 66449-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HVCN1 Monoclonal antibody

HVCN1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66449-1-Ig

产品说明书

CloneNo. 1E4C4

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

HV1, HVCN1, VSOP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inRaji cells, HeLa cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66449-1-Ig targets HVCN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 HVCN1 fusion protein Ag5350 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hydrogen voltage-gated channel 1
别名 HV1, HVCN1, VSOP
计算分子量 273 aa, 32 kDa
观测分子量 28-35 kDa, 40 kDa, 60 kDa
GenBank蛋白编号BC032672
基因名称 HVCN1
Gene ID (NCBI) 84329
RRIDAB_2881818
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96D96
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.

实验方案

Product Specific Protocols
WB protocol for HVCN1 antibody 66449-1-IgDownload protocol
IHC protocol for HVCN1 antibody 66449-1-IgDownload protocol
IF protocol for HVCN1 antibody 66449-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols