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HVCN1 Monoclonal antibody, PBS Only

HVCN1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4C4

Cat no : 66449-1-PBS

Print datasheet

Synonyms

HV1, HVCN1, VSOP

验证数据展示

  • WB analysis of Raji using 66449-1-Ig (same clone as 66449-1-PBS)

    WB analysis of Raji using 66449-1-Ig (same clone as 66449-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66449-1-Ig (HVCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    IF Staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using HVCN1 antibody (66449-1-Ig, Clone: 1E4C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    IHC staining of human tonsillitis using 66449-1-Ig (same clone as 66449-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66449-1-Ig (HVCN1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66449-1-PBS in a different storage buffer formulation.

产品信息

66449-1-PBS targets HVCN1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen HVCN1 fusion protein Ag5350 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hydrogen voltage-gated channel 1
Synonyms HV1, HVCN1, VSOP
Calculated Molecular Weight 273 aa, 32 kDa
Observed Molecular Weight 28-35 kDa, 40 kDa, 60 kDa
GenBank Accession NumberBC032672
Gene Symbol HVCN1
Gene ID (NCBI) 84329
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ96D96
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HVCN1, also named as VSOP and HV1, Belongs to the hydrogen channel family. HVCN1 mediates the voltage-dependent proton permeability of excitable membranes. It forms a proton-selective channel through which protons may pass in accordance with their electrochemical gradient. Proton efflux, HVCN1 is accompanied by membrane depolarization, facilitates acute production of reactive oxygen species in phagocytosis. HVCN1, the voltage-sensitive proton channel, is present in human sperm and is an important regulator of the functional maturation of sperm. HVCN1 has four isoforms with MW 28-32 kDa or 40 kDa (modification). It has a dimer form with MW 60 kDa.