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  • Featured Product
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Thioredoxin Monoclonal antibody

Thioredoxin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F3C1

Cat no : 66475-1-Ig

Print datasheet

Synonyms

ADF, ATL derived factor, SASP, thioredoxin, TRDX, TRX, TRX1, TXN

验证数据展示

  • WB analysis using 66475-1-Ig

    WB analysis using 66475-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66475-1-Ig (TXN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human ovary tumor using 66475-1-Ig

    IHC staining of human ovary tumor using 66475-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66475-1-Ig (Thioredoxin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TXN antibody included
  • IF Staining of HepG2 using 66475-1-Ig

    IF Staining of HepG2 using 66475-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Thioredoxin antibody (66475-1-Ig, Clone: 1F3C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66475-1-Ig

    WB analysis using 66475-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66475-1-Ig (Thioredoxin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66475-1-Ig

    WB analysis of HeLa using 66475-1-Ig

    WB result of Thioredoxin antibody (66475-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thioredoxin transfected HeLa cells.

经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells, A549 cells, Jurkat cells, HEK-293 cells, K-562 cells
Positive IHC detected inhuman ovary tumor tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TXN Ready-To-Use IHC Kit. TXN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66475-1-Ig targets Thioredoxin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Thioredoxin fusion protein Ag6355 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name thioredoxin
Synonyms ADF, ATL derived factor, SASP, thioredoxin, TRDX, TRX, TRX1, TXN
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC003377
Gene Symbol TXN
Gene ID (NCBI) 7295
RRIDAB_2881841
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10599
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

实验方案

Product Specific Protocols
WB protocol for Thioredoxin antibody 66475-1-IgDownload protocol
IHC protocol for Thioredoxin antibody 66475-1-IgDownload protocol
IF protocol for Thioredoxin antibody 66475-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Redox Biol

Guiding bar motif of thioredoxin reductase 1 modulates enzymatic activity and inhibitor binding by communicating with the co-factor FAD and regulating the flexible C-terminal redox motif

Authors - Wuyang Shi
View Article