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验证数据展示

  • IF Staining of HepG2 using CL594-66475

    IF Staining of HepG2 using CL594-66475

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594-conjugated TXN antibody (CL594-66475, Clone: 1F3C1 ) at dilution of 1:1000, C488-Phalloidin (green).

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  • KD/KO验证

CoraLite®594-conjugated TXN Monoclonal antibody

TXN Monoclonal Antibody for IF/ICC
Cat No. CL594-66475

产品说明书

CloneNo. 1F3C1

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF/ICC

别名

ADF, ATL derived factor, DKFZp686B1993, SASP, thioredoxin, TRDX, TRX, TRX1, TXN

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TXN Ready-To-Use IHC Kit. TXN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66475-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66475 targets TXN in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 TXN fusion protein Ag6355 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 thioredoxin
别名 ADF, ATL derived factor, DKFZp686B1993, SASP, thioredoxin, TRDX, TRX, TRX1, TXN
计算分子量 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC003377
基因名称 TXN
Gene ID (NCBI) 7295
RRIDAB_2883587
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10599
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.

实验方案

Product Specific Protocols
IF protocol for CL594 TXN antibody CL594-66475Download protocol
Standard Protocols
Click here to view our Standard Protocols