- Featured Product
- KD/KO Validated
Thioredoxin Monoclonal antibody
Thioredoxin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1F3C1
验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, MCF-7 cells, HeLa cells, HepG2 cells, A549 cells, Jurkat cells, HEK-293 cells, K-562 cells |
Positive IHC detected in | human ovary tumor tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
66475-1-Ig targets Thioredoxin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human |
Cited Reactivity | human |
Immunogen | Thioredoxin fusion protein Ag6355 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | thioredoxin |
Synonyms | ADF, ATL derived factor, SASP, thioredoxin, TRDX, TRX, TRX1, TXN |
Calculated Molecular Weight | 12 kDa |
Observed Molecular Weight | 12 kDa |
GenBank Accession Number | BC003377 |
Gene Symbol | TXN |
Gene ID (NCBI) | 7295 |
RRID | AB_2881841 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P10599 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Thioredoxin antibody 66475-1-Ig | Download protocol |
IHC protocol for Thioredoxin antibody 66475-1-Ig | Download protocol |
IF protocol for Thioredoxin antibody 66475-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Redox Biol Guiding bar motif of thioredoxin reductase 1 modulates enzymatic activity and inhibitor binding by communicating with the co-factor FAD and regulating the flexible C-terminal redox motif | ||
Biol Trace Elem Res Pharmacological Inhibition of TXNRD1 by a Small Molecule Flavonoid Butein Overcomes Cisplatin Resistance in Lung Cancer Cells |