• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66480-1-Ig

    WB analysis using 66480-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66480-1-Ig (ARHGDIA,aGDI antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 66480-1-Ig

    WB analysis of HEK-293 using 66480-1-Ig

    WB result of RhoGDI antibody (66480-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RhoGDI transfected HEK-293 cells.

  • Featured Product
  • KD/KO验证

RhoGDI Monoclonal antibody

RhoGDI Monoclonal Antibody for WB, ELISA
Cat No. 66480-1-Ig

产品说明书

CloneNo. 1B1G9

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, ELISA

别名

aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, C6 cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, NIH/3T3 cells
Planning an IHC experiment? We recommend our IHCeasy ARHGDIA Ready-To-Use IHC Kit. ARHGDIA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66480-1-Ig targets RhoGDI in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原 RhoGDI fusion protein Ag0791 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Rho GDP dissociation inhibitor (GDI) alpha
别名 aGDI, ARHGDIA, GDIA1, Rho GDI 1, Rho GDI alpha, RHOGDI, RHOGDI 1
计算分子量 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC005851
基因名称 RhoGDI
Gene ID (NCBI) 396
RRIDAB_2881846
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP52565
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rho GDP-dissociation inhibitor (RhoGDI), also called ARHGDIA, is one member of ARHGDIs, which is ubiquitously expressed and interacts with several Rho GTPases, mainly including RhoA, Rac1, and Cdc42. RhoGDI can inhibit nucleotide exchange and membrane association to downregulate the activities of Rho family GTPase. It is overexpressed in various human cancers. Activity of this protein is important in a variety of cellular processes, and it is overexpressed in various human cancers. Mutations in RhoGDI have been found in individuals with nephrotic syndrome, type 8.

实验方案

Product Specific Protocols
WB protocol for RhoGDI antibody 66480-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols