验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, HEK-293 cells, rat brain tissue, mouse brain tissue |
| Positive IF/ICC detected in | Neuro-2a cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:900-1:3600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66488-1-Ig targets VAMP3/Cellubrevin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig |
| 文献引用反应性 | human |
| 免疫原 | VAMP3/Cellubrevin fusion protein Ag1156 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vesicle-associated membrane protein 3 (cellubrevin) |
| 别名 | VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB |
| 计算分子量 | 11 kDa |
| 观测分子量 | 17 kDa |
| GenBank蛋白编号 | BC005941 |
| 基因名称 | VAMP3 |
| Gene ID (NCBI) | 9341 |
| RRID | AB_2881853 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q15836 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VAMP3, also named cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VAMP3/Cellubrevin antibody 66488-1-Ig | Download protocol |
| IF protocol for VAMP3/Cellubrevin antibody 66488-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
