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VAMP3/Cellubrevin Monoclonal antibody, PBS Only

VAMP3/Cellubrevin Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D10D10

Cat no : 66488-1-PBS

Print datasheet

Synonyms

VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB

验证数据展示

  • WB analysis of pig brain using 66488-1-Ig (same clone as 66488-1-PBS)

    WB analysis of pig brain using 66488-1-Ig (same clone as 66488-1-PBS)

    Pig brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 66488-1-Ig (same clone as 66488-1-PBS)

    IF Staining of Neuro-2a using 66488-1-Ig (same clone as 66488-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using VAMP3/Cellubrevin antibody (66488-1-Ig, Clone: 1D10D10 ) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy VAMP3/Cellubrevin Ready-To-Use IHC Kit. VAMP3/Cellubrevin primary antibody included.

产品信息

66488-1-PBS targets VAMP3/Cellubrevin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen VAMP3/Cellubrevin fusion protein Ag1156 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name vesicle-associated membrane protein 3 (cellubrevin)
Synonyms VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC005941
Gene Symbol VAMP3
Gene ID (NCBI) 9341
RRIDAB_2881853
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15836
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAMP3, also named cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.