• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66540-1-Ig (same clone as 66540-1-PBS)

    WB analysis using 66540-1-Ig (same clone as 66540-1-PBS)

    Various tissue were subjected to SDS PAGE followed by western blot with 66540-1-Ig (Calponin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

  • IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

    IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

  • IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

    IHC staining of human myofibroblastoma using 66540-1-Ig (same clone as 66540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66540-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50017-1

    Sandwich ELISA standard curve of MP50017-1

    Sandwich ELISA standard curve ofMP50017-1, Calponin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66540-2-PBS. Detection antibody: HRP-conjugated 66540-1-PBS. Standard: Ag21384. Range: 0.313-20 ng/mL.

Calponin Monoclonal antibody, PBS Only (Detector)

Calponin Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA
Cat No. 66540-1-PBS

产品说明书

CloneNo. 1A11E5

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IHC, Sandwich ELISA, Indirect ELISA

别名

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

66540-1-PBS targets Calponin as part of a matched antibody pair:

MP50017-1: 66540-2-PBS capture and 66540-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 Human
免疫原 Calponin fusion protein Ag21384 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 calponin 1, basic, smooth muscle
别名 Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC
计算分子量 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC022015
基因名称 Calponin
Gene ID (NCBI) 1264
RRIDAB_2881902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP51911
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.