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SIRT4 Monoclonal antibody
SIRT4 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
1D5A2
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NCCIT cell, PC-3 cells, L02 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:80000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66543-1-Ig targets SIRT4 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | SIRT4 fusion protein Ag17347 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae) |
| Synonyms | SIR2 like protein 4, SIR2L4, SIRT4 |
| Calculated Molecular Weight | 314 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC109319 |
| Gene Symbol | SIRT4 |
| Gene ID (NCBI) | 23409 |
| RRID | AB_2881905 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y6E7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRT4 antibody 66543-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
ACS Nano Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer | ||
Cell Death Differ SIRT1 coordinates with the CRL4B complex to regulate pancreatic cancer stem cells to promote tumorigenesis.
| ||
Cancer Lett SETD8 potentiates constitutive ERK1/2 activation via epigenetically silencing DUSP10 expression in pancreatic cancer. | ||
iScience Sirt6 regulates the proliferation of neural precursor cells and cortical neurogenesis in mice | ||
Int Immunopharmacol Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma | ||
Lab Invest Targeting SIRT4/TET2 Signaling Alleviates Human Keratinocyte Senescence by Reducing 5-hmC Loss |
