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  • Featured Product
  • KD/KO Validated

SIRT4 Monoclonal antibody, PBS Only

SIRT4 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5A2

Cat No : 66543-1-PBS

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Synonyms

SIR2 like protein 4, SIR2L4, SIRT4

验证数据展示

  • WB analysis of HeLa using 66543-1-Ig (same clone as 66543-1-PBS)

    WB analysis of HeLa using 66543-1-Ig (same clone as 66543-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66543-1-Ig (SIRT4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66543-1-PBS in a different storage buffer formulation.

产品信息

66543-1-PBS targets SIRT4 in WB, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen SIRT4 fusion protein Ag17347 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
Synonyms SIR2 like protein 4, SIR2L4, SIRT4
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC109319
Gene Symbol SIRT4
Gene ID (NCBI) 23409
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6E7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT4, also named as NAD-dependent protein lipoamidase sirtuin-4, mitochondria, is a 314 amino acid protein, which belongs to the sirtuin family. Class II subfamily. SIRT4 is detected in vascular smooth muscle and striated muscle. SIRT4 is detected in insulin-producing beta-cells in pancreas islets of Langerhans. SIRT4 Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. It catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications and inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 expression is down-regulated in a number of cancers, while overexpression reduces cell proliferation, transformation, and tumor development.