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RANKL Monoclonal antibody
RANKL Monoclonal Antibody for IF/ICC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
IF/ICC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3F2E1
验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, HeLa cells, U2OS cells, HUVEC cells, NCCIT cells, human spleen tissue, DC2.4 cells, JAR cells, RAW 264.7 cells |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66610-1-Ig targets RANKL in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IF/ICC, WB, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | RANKL fusion protein Ag19975 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | tumor necrosis factor (ligand) superfamily, member 11 |
Synonyms | CD254, hRANKL2, ODF, OPGL, OPTB2, Osteoprotegerin ligand, RANKL, sOdf, TNFSF11, TRANCE |
Calculated Molecular Weight | 317 aa, 35 kDa |
Observed Molecular Weight | 35-38 kDa |
GenBank Accession Number | BC074890 |
Gene Symbol | TNFSF11 |
Gene ID (NCBI) | 8600 |
RRID | AB_2881970 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O14788 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for RANKL antibody 66610-1-Ig | Download protocol |
IF protocol for RANKL antibody 66610-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Cancer Gut microbiota deficiency ameliorates multiple myeloma and myeloma-related bone disease by Th17 cells in mice models | ||
Cell Death Dis RANK promotes colorectal cancer migration and invasion by activating the Ca2+-calcineurin/NFATC1-ACP5 axis. | ||
Open Life Sci Effect of periostin on bone metabolic and autophagy factors during tooth eruption in mice | ||
J Periodontal Res Role of Piezo1 in modulating the RANKL/OPG ratio in mouse osteoblast cells exposed to Porphyromonas gingivalis lipopolysaccharide and mechanical stress | ||
Cell Metab Gut microbial alterations in arginine metabolism determine bone mechanical adaptation |