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  • Featured Product
  • KD/KO Validated

TNFSF11/RANKL Monoclonal antibody, PBS Only (Capture)

TNFSF11/RANKL Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F2E1

Cat no : 66610-1-PBS

Print datasheet

Synonyms

TNFSF11, TNF-related activation-induced cytokine, Receptor activator of nuclear factor kappa-B ligand, CD254, CD 254

验证数据展示

  • WB analysis using 66610-1-Ig (same clone as 66610-1-PBS)

    WB analysis using 66610-1-Ig (same clone as 66610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66610-1-Ig (RANKL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66610-1-Ig (same clone as 66610-1-PBS)

    WB analysis of HeLa using 66610-1-Ig (same clone as 66610-1-PBS)

    WB result of RANKL antibody (66610-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RANKL transfected HeLa cells. This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66610-1-Ig (same clone as 66610-1-PBS)

    IF Staining of MCF-7 using 66610-1-Ig (same clone as 66610-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RANKL antibody (66610-1-Ig, Clone: 3F2E1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66610-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50526-1

    Cytometric bead array standard curve of MP50526-1

    Cytometric bead array standard curve of MP50526-1, RANKL Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66610-1-PBS. Detection antibody: 66610-2-PBS. Standard:Ag19975. Range: 1.563-100 ng/mL.

产品信息

66610-1-PBS targets TNFSF11/RANKL as part of a matched antibody pair:

MP50526-1: 66610-1-PBS capture and 66610-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TNFSF11/RANKL fusion protein Ag19975 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 11
Synonyms TNFSF11, TNF-related activation-induced cytokine, Receptor activator of nuclear factor kappa-B ligand, CD254, CD 254
Calculated Molecular Weight 317 aa, 35 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC074890
Gene Symbol RANKL
Gene ID (NCBI) 8600
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14788
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNFSF11 also known as RANKL, is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL is a polypeptide of 217 amino acids that exerts its biological activity both in a transmembrane form of about 40-45 kDa and in soluble one of 31 kDa (PMID: 15308315). The membrane-bound RANKL (mRANKL) is cleaved into a sRANKL by the metalloprotease-disintegrin TNF-alpha convertase (TACE) or a related metalloprotease (MP). RANKL induces osteoclast formation through its receptor, RANK, which transduces signals by recruiting adaptor molecules, such as the TNF receptor-associated factor (TRAF) family of proteins. RANKL was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. RANKL was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis.