验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, L02 cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
产品信息
66619-1-Ig targets Adiponectin receptor 1 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | Adiponectin receptor 1 fusion protein Ag18187 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | adiponectin receptor 1 |
| 别名 | ACDCR1, Adiponectin receptor 1, Adiponectin receptor protein 1, ADIPOR1, CGI 45, CGI45, PAQR1, TESBP1A |
| 计算分子量 | 375 aa, 43 kDa |
| 观测分子量 | 43 kDa |
| GenBank蛋白编号 | BC010743 |
| 基因名称 | Adiponectin receptor 1 |
| Gene ID (NCBI) | 51094 |
| RRID | AB_2881979 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q96A54 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin have been identified: adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). They are seven-transmembrane domain receptors and mediate increased AMPK, MAPK and PPARA ligand activity in response to adiponectin. AdipoR1 is found in many tissues, being particularly abundant in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver (PMID: 24417942). AdipoR1 has a high affinity for globular adiponectin and a very low affinity for full-length adiponectin. AdipoR2 has an intermediate affinity for both forms. The human ADIPOR1 gene maps to chromosome 1q32.1, and encodes a 375-amino acid protein with a molecular weight of 43 kDa. This antibody was raised against 1-72aa of human AdipoR1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Adiponectin receptor 1 antibody 66619-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Food Funct A flavonoid-rich Smilax china L. extract prevents obesity by upregulating the adiponectin-receptor/AMPK signalling pathway and modulating the gut microbiota in mice. | ||
Food Funct Quercetin regulates skeletal muscle fiber type switching via adiponectin signaling. | ||
Biol Open A stem cell based in vitro model of NAFLD enables the analysis of patient specific individual metabolic adaptations in response to a high fat diet and AdipoRon interference. | ||
Anim Biotechnol Leucine regulates porcine muscle fiber type transformation via adiponectin signaling pathway. | ||
Anim Biotechnol Effect of dietary leucine supplementation on skeletal muscle fiber type transformation in weaning piglets | ||
J Agric Food Chem Probiotic Yogurt Alleviates High-Fat Diet-Induced Lipid Accumulation and Insulin Resistance in Mice via the Adiponectin Pathway |