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ANGPTL8/Betatrophin Monoclonal antibody

ANGPTL8/Betatrophin Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2H9F9

Cat no : 66641-1-Ig

Print datasheet

Synonyms

Angiopoietin-like protein 8, ANGPTL8, Betatrophin, C19orf80, Lipasin, LOC55908, PRO1185, PVPA599, td26

验证数据展示

  • WB analysis of human adipose using 66641-1-Ig

    WB analysis of human adipose using 66641-1-Ig

    human adipose tissue were subjected to SDS PAGE followed by western blot with 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66641-1-Ig

    IHC staining of human liver using 66641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:400 (under 40x lens).

  • IHC staining of human liver using 66641-1-Ig

    IHC staining of human liver using 66641-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66641-1-Ig (ANGPTL8/Betatrophin antibody) at dilution of 1:400 (under 10x lens).

  • IF Staining of human liver using 66641-1-Ig

    IF Staining of human liver using 66641-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using ANGPTL8/Betatrophin antibody (66641-1-Ig, Clone: 2H9F9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman adipose tissue
Positive IHC detected inhuman liver tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver tissue
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66641-1-Ig targets ANGPTL8/Betatrophin in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen ANGPTL8/Betatrophin fusion protein Ag20708 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hepatocellular carcinoma-associated gene TD26
Synonyms Angiopoietin-like protein 8, ANGPTL8, Betatrophin, C19orf80, Lipasin, LOC55908, PRO1185, PVPA599, td26
Calculated Molecular Weight 251 aa, 28 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC146552
Gene Symbol ANGPTL8
Gene ID (NCBI) 55908
RRIDAB_2882000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6UXH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
IHC protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
IF protocol for ANGPTL8/Betatrophin antibody 66641-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Br J Pharmacol

Sodium rutin extends lifespan and health span in mice including positive impacts on liver health.

Authors - Shuoshuo Li
View Article