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验证数据展示

  • WB analysis of Raji using 66659-1-Ig (same clone as 66659-1-PBS)

    WB analysis of Raji using 66659-1-Ig (same clone as 66659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66659-1-Ig (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66659-1-PBS in a different storage buffer formulation.

  • IHC staining of Human tonsillitis using 66659-1-Ig (same clone as 66659-1-PBS)

    IHC staining of Human tonsillitis using 66659-1-Ig (same clone as 66659-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66659-1-Ig (POU2AF1 antibody) at dilution of 1:1700 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66659-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51058-1

    Cytometric bead array standard curve of MP51058-1

    Cytometric bead array standard curve of MP51058-1, POU2AF1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66659-2-PBS. Detection antibody: 66659-1-PBS. Standard:Ag23613. Range: 0.781-200 ng/mL.

  • Cytometric bead array standard curve of MP51058-2

    Cytometric bead array standard curve of MP51058-2

    Cytometric bead array standard curve of MP51058-2, POU2AF1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66659-1-PBS. Detection antibody: 66659-3-PBS. Standard:Ag23613. Range: 0.781-200 ng/mL.

POU2AF1 Monoclonal antibody, PBS Only (Capture/Detector)

POU2AF1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 66659-1-PBS

产品说明书

CloneNo. 3C5A7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Cytometric bead array, Indirect ELISA

别名

OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66659-1-PBS targets POU2AF1 as part of a matched antibody pair:

MP51058-1: 66659-2-PBS capture and 66659-1-PBS detection (validated in Cytometric bead array)

MP51058-2: 66659-1-PBS capture and 66659-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 POU2AF1 fusion protein Ag23613 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 POU class 2 associating factor 1
别名 OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1
计算分子量 256 aa, 27 kDa
观测分子量 35 kDa
GenBank蛋白编号BC032549
基因名称 POU2AF1
Gene ID (NCBI) 5450
ENSEMBL Gene IDENSG00000110777
RRIDAB_2882016
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16633
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.