验证数据展示
经过测试的应用
Positive WB detected in | Raji cells, Ramos cells, Daudi cells |
Positive IHC detected in | Human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:850-1:3400 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66659-1-Ig targets POU2AF1 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
经测试反应性 | human |
免疫原 | POU2AF1 fusion protein Ag23613 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | POU class 2 associating factor 1 |
别名 | OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1 |
计算分子量 | 256 aa, 27 kDa |
观测分子量 | 35 kDa |
GenBank蛋白编号 | BC032549 |
基因名称 | POU2AF1 |
Gene ID (NCBI) | 5450 |
ENSEMBL Gene ID | ENSG00000110777 |
RRID | AB_2882016 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q16633 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.
实验方案
Product Specific Protocols | |
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WB protocol for POU2AF1 antibody 66659-1-Ig | Download protocol |
IHC protocol for POU2AF1 antibody 66659-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |