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POU2AF1 Monoclonal antibody, PBS Only (Capture/Detector)

POU2AF1 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C5A7

Cat no : 66659-1-PBS

Print datasheet

Synonyms

OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1

验证数据展示

  • WB analysis of Raji using 66659-1-Ig (same clone as 66659-1-PBS)

    WB analysis of Raji using 66659-1-Ig (same clone as 66659-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66659-1-Ig (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66659-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51058-1

    Cytometric bead array standard curve of MP51058-1

    Cytometric bead array standard curve of MP51058-1, POU2AF1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66659-2-PBS. Detection antibody: 66659-1-PBS. Standard:Ag23613. Range: 0.781-200 ng/mL.

  • Cytometric bead array standard curve of MP51058-2

    Cytometric bead array standard curve of MP51058-2

    Cytometric bead array standard curve of MP51058-2, POU2AF1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66659-1-PBS. Detection antibody: 66659-3-PBS. Standard:Ag23613. Range: 0.781-200 ng/mL.

产品信息

66659-1-PBS targets POU2AF1 as part of a matched antibody pair:

MP51058-1: 66659-2-PBS capture and 66659-1-PBS detection (validated in Cytometric bead array)

MP51058-2: 66659-1-PBS capture and 66659-3-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen POU2AF1 fusion protein Ag23613 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name POU class 2 associating factor 1
Synonyms OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1
Calculated Molecular Weight 256 aa, 27 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC032549
Gene Symbol POU2AF1
Gene ID (NCBI) 5450
ENSEMBL Gene IDENSG00000110777
RRIDAB_2882016
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16633
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.