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验证数据展示

  • FC experiment of Ramos using CL488-66659

    FC experiment of Ramos using CL488-66659

    1x10^6 Ramos cells were intracellularly stained with 0.2 ug Coralite® Plus 488 Pou2af1 Monoclonal Antibody (CL488-66659, Clone:3C5A7)(red), or 0.2 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated POU2AF1 Monoclonal antibody

POU2AF1 Monoclonal Antibody for FC (Intra)
Cat No. CL488-66659

Print datasheet

CloneNo. 3C5A7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

FC (Intra)

Synonyms

OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inRamos cells
For other applications, we recommend the unconjugated version of this antibody, 66659-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66659 targets POU2AF1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen POU2AF1 fusion protein Ag23613 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name POU class 2 associating factor 1
Synonyms OCAB, OBF1, BOB-1, BOB1, B-cell-specific coactivator OBF-1
Calculated Molecular Weight 256 aa, 27 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC032549
Gene Symbol POU2AF1
Gene ID (NCBI) 5450
ENSEMBL Gene IDENSG00000110777
RRIDAB_3672919
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16633
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 POU2AF1 antibody CL488-66659Download protocol
Standard Protocols
Click here to view our Standard Protocols