• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HeLa using 66676-1-Ig

    WB analysis of HeLa using 66676-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66676-1-Ig

    IF Staining of HepG2 using 66676-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66676-1-Ig (HMG20A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

HMG20A Monoclonal antibody

HMG20A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66676-1-Ig

产品说明书

CloneNo. 1B10F12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

1B10F12, high mobility group 20A, HMG box containing protein 20A, HMG box-containing protein 20A, HMG domain-containing protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, NCCIT cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66676-1-Ig targets HMG20A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 HMG20A fusion protein Ag2718 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 high-mobility group 20A
别名 1B10F12, high mobility group 20A, HMG box containing protein 20A, HMG box-containing protein 20A, HMG domain-containing protein 1
计算分子量 347 aa, 40 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC021959
基因名称 HMG20A
Gene ID (NCBI) 10363
RRIDAB_2882030
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NP66
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation

实验方案

Product Specific Protocols
WB protocol for HMG20A antibody 66676-1-IgDownload protocol
IF protocol for HMG20A antibody 66676-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols