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HMG20A Monoclonal antibody, PBS Only

HMG20A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B10F12

Cat no : 66676-1-PBS

Print datasheet

Synonyms

FLJ10739, high mobility group 20A, HMG box containing protein 20A, HMG20A, HMGX1, HMGXB1

验证数据展示

  • WB analysis of HeLa using 66676-1-Ig (same clone as 66676-1-PBS)

    WB analysis of HeLa using 66676-1-Ig (same clone as 66676-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66676-1-Ig (same clone as 66676-1-PBS)

    IF Staining of HepG2 using 66676-1-Ig (same clone as 66676-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66676-1-Ig (HMG20A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

产品信息

66676-1-PBS targets HMG20A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen HMG20A fusion protein Ag2718 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name high-mobility group 20A
Synonyms FLJ10739, high mobility group 20A, HMG box containing protein 20A, HMG20A, HMGX1, HMGXB1
Calculated Molecular Weight 347 aa, 40 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC021959
Gene Symbol HMG20A
Gene ID (NCBI) 10363
RRIDAB_2882030
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9NP66
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation