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验证数据展示

  • WB analysis of HeLa using 66676-1-Ig (same clone as 66676-1-PBS)

    WB analysis of HeLa using 66676-1-Ig (same clone as 66676-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66676-1-Ig (HMG20A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66676-1-Ig (same clone as 66676-1-PBS)

    IF Staining of HepG2 using 66676-1-Ig (same clone as 66676-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66676-1-Ig (HMG20A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66676-1-PBS in a different storage buffer formulation.

HMG20A Monoclonal antibody, PBS Only

HMG20A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66676-1-PBS

产品说明书

CloneNo. 1B10F12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

1B10F12, high mobility group 20A, HMG box containing protein 20A, HMG box-containing protein 20A, HMG domain-containing protein 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66676-1-PBS targets HMG20A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HMG20A fusion protein Ag2718 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 high-mobility group 20A
别名 1B10F12, high mobility group 20A, HMG box containing protein 20A, HMG box-containing protein 20A, HMG domain-containing protein 1
计算分子量 347 aa, 40 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC021959
基因名称 HMG20A
Gene ID (NCBI) 10363
RRIDAB_2882030
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9NP66
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The HMG (high mobility group) proteins are nonhistone chromosomal proteins that is present in almost all eukaryotic cells, and it functions to stabilize nucleosome formation and acts as a transcription-factor-like protein that regulates the expression of several genes. HMG20A has a role in the initiation of neuronal differentiation by activating REST-responsive genes, and it also nvolves in the recruitment of the histone methyltransferase MLL and consequent increased methylation of histone H3 lysine 4. HMG20A (for inhibitor of BRAF35) overcomes the repressive effects of the neuronal silencer REST and activates REST-responsive genes through the modulation of histone methylation