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验证数据展示

  • IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA/FOLH1 antibody included
  • WB analysis of LNCaP using 66678-1-Ig (same clone as 66678-1-PBS)

    WB analysis of LNCaP using 66678-1-Ig (same clone as 66678-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • WB analysis using 66678-1-Ig (same clone as 66678-1-PBS)

    WB analysis using 66678-1-Ig (same clone as 66678-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA/FOLH1 antibody included
  • IF Staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    IF Staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA/FOLH1 antibody included
  • IF Staining of PC-3 using 66678-1-Ig (same clone as 66678-1-PBS)

    IF Staining of PC-3 using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51112-1

    Cytometric bead array standard curve of MP51112-1

    Cytometric bead array standard curve of MP51112-1, FOLH1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66678-2-PBS. Detection antibody: 66678-1-PBS. Standard:Ag16594. Range: 1.563-100 ng/mL.

PSMA/GCPII Monoclonal antibody, PBS Only (Detector)

PSMA/GCPII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66678-1-PBS

产品说明书

CloneNo. 3G4E12

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

别名

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PSMA/FOLH1 Ready-To-Use IHC Kit. PSMA/FOLH1 primary antibody included.

产品信息

66678-1-PBS targets PSMA/GCPII as part of a matched antibody pair:

MP51112-1: 66678-2-PBS capture and 66678-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 PSMA/GCPII fusion protein Ag16594 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 folate hydrolase (prostate-specific membrane antigen) 1
别名 FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP
计算分子量 719 aa, 81 kDa
观测分子量 100-120 kDa
GenBank蛋白编号BC025672
基因名称 PSMA
Gene ID (NCBI) 2346
RRIDAB_2882032
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ04609
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.