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PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3G4E12

Cat no : 66678-1-Ig

Print datasheet

Synonyms

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

验证数据展示

  • IHC staining of human prostate cancer using 66678-1-Ig

    IHC staining of human prostate cancer using 66678-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA/FOLH1 antibody included
  • WB analysis of LNCaP using 66678-1-Ig

    WB analysis of LNCaP using 66678-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 66678-1-Ig

    IHC staining of human prostate cancer using 66678-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PSMA/FOLH1 antibody included
  • IF Staining of human prostate cancer using 66678-1-Ig

    IF Staining of human prostate cancer using 66678-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of PC-3 using 66678-1-Ig

    IF Staining of PC-3 using 66678-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, rat kidney tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman prostate cancer tissue, PC-3 cells
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA/FOLH1 Ready-To-Use IHC Kit. PSMA/FOLH1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:4000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66678-1-Ig targets PSMA/GCPII in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, rat
Cited Reactivityhuman
Immunogen PSMA/GCPII fusion protein Ag16594 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Synonyms FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC025672
Gene Symbol PSMA
Gene ID (NCBI) 2346
RRIDAB_2882032
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04609
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
IHC protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
IF protocol for PSMA/GCPII antibody 66678-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Cancer cell's neuroendocrine feature can be acquired through cell-cell fusion during cancer-neural stem cell interaction.

Authors - Liyuan Yin
View Article