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验证数据展示

  • IF Staining of human prostate cancer using CL488-66678

    IF Staining of human prostate cancer using CL488-66678

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • IF Staining of human prostate cancer using CL488-66678

    IF Staining of human prostate cancer using CL488-66678

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • IF Staining of PC-3 using CL488-66678

    IF Staining of PC-3 using CL488-66678

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-66678

产品说明书

CloneNo. 3G4E12

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

IF/ICC, IF-P

别名

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman prostate cancer tissue, PC-3 cells
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA/FOLH1 Ready-To-Use IHC Kit. PSMA/FOLH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66678-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66678 targets PSMA/GCPII in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, rat
免疫原 PSMA/GCPII fusion protein Ag16594 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 folate hydrolase (prostate-specific membrane antigen) 1
别名 FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP
计算分子量 719 aa, 81 kDa
观测分子量 100-120 kDa
GenBank蛋白编号BC025672
基因名称 PSMA
Gene ID (NCBI) 2346
RRIDAB_2919356
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ04609
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PSMA/GCPII antibody CL488-66678Download protocol
Standard Protocols
Click here to view our Standard Protocols