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CoraLite® Plus 488-conjugated PSMA/GCPII Monoclonal antibody

PSMA/GCPII Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G4E12

Cat no : CL488-66678

Print datasheet

Synonyms

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

验证数据展示

  • IF Staining of human prostate cancer using CL488-66678

    IF Staining of human prostate cancer using CL488-66678

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • IF Staining of human prostate cancer using CL488-66678

    IF Staining of human prostate cancer using CL488-66678

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:200.

  • IF Staining of PC-3 using CL488-66678

    IF Staining of PC-3 using CL488-66678

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 PSMA/GCPII antibody (CL488-66678, Clone: 3G4E12 ) at dilution of 1:100.

经过测试的应用

Positive IF-P detected inhuman prostate cancer tissue, PC-3 cells
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy PSMA/FOLH1 Ready-To-Use IHC Kit. PSMA/FOLH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66678-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66678 targets PSMA/GCPII in IF/ICC, IF-P applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, rat
Immunogen PSMA/GCPII fusion protein Ag16594 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Synonyms FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC025672
Gene Symbol PSMA
Gene ID (NCBI) 2346
RRIDAB_2919356
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ04609
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PSMA/GCPII antibody CL488-66678Download protocol
Standard Protocols
Click here to view our Standard Protocols