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PSMA/GCPII Monoclonal antibody, PBS Only

PSMA/GCPII Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G4E12

Cat no : 66678-1-PBS

Print datasheet

Synonyms

FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP

验证数据展示

  • IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA/FOLH1 antibody included
  • WB analysis of LNCaP using 66678-1-Ig (same clone as 66678-1-PBS)

    WB analysis of LNCaP using 66678-1-Ig (same clone as 66678-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 66678-1-Ig (FOLH1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    IHC staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66678-1-Ig (PSMA/GCPII antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PSMA/FOLH1 antibody included
  • IF Staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    IF Staining of human prostate cancer using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 66678-1-Ig (same clone as 66678-1-PBS)

    IF Staining of PC-3 using 66678-1-Ig (same clone as 66678-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using PSMA/GCPII antibody (66678-1-Ig, Clone: 3G4E12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66678-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PSMA/FOLH1 Ready-To-Use IHC Kit. PSMA/FOLH1 primary antibody included.

产品信息

66678-1-PBS targets PSMA/GCPII in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen PSMA/GCPII fusion protein Ag16594 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name folate hydrolase (prostate-specific membrane antigen) 1
Synonyms FOLH1, PSMA, FOLH, Folate hydrolase 1, FGCP
Calculated Molecular Weight 719 aa, 81 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC025672
Gene Symbol PSMA
Gene ID (NCBI) 2346
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ04609
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PSMA(Prostate-specific membrane antigen) is also named as FOLH1, FOLH, NAALAD1, PSM and belongs to the peptidase M28 family. PSMA is a 100-120 kDa integral transmembrane glycoprotein, considered to be a highly specific marker of the prostate gland, and has successfully been used as a marker of circulating prostatic epithelial cells(PMID:10074909; 15680901). It is involved in conversion of the major neurotransmitter (NAAG) to NAA and free glutamate. It has 8 isoforms produced by alternative splicing.