BTC Monoclonal antibody
BTC Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2H5E8
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, NCCIT cells, A549 cells, MDA-MB-231 cells, T-47D cells, PC-3 cells, BxPC-3 cells, DU 145 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
66683-1-Ig targets BTC in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | BTC fusion protein Ag26724 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | betacellulin |
| Synonyms | betacellulin, BTC, Probetacellulin |
| Calculated Molecular Weight | 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC011618 |
| Gene Symbol | BTC |
| Gene ID (NCBI) | 685 |
| RRID | AB_2882037 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35070 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, binds and activates ErbB1 and ErbB4 homodimers. BTC is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. BTC was expressed in tumors and involved in tumor growth progression.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for BTC antibody 66683-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Invest Dermatol LPCAT1 promotes cutaneous squamous cell carcinoma via EGFR-mediated AKT/p38MAPK signaling pathways. | ||
Front Genet BTC as a Novel Biomarker Contributing to EMT via the PI3K-AKT Pathway in OSCC. |
