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CoraLite® Plus 488-conjugated BTC Monoclonal antibody

BTC Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H5E8

Cat no : CL488-66683

Print datasheet

Synonyms

betacellulin, BTC, Probetacellulin

验证数据展示

  • WB analysis using CL488-66683

    WB analysis using CL488-66683

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66683 (BTC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, MDA-MB-231 cells
For other applications, we recommend the unconjugated version of this antibody, 66683-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66683 targets BTC in WB applications and shows reactivity with Human samples.

Tested Applications WB Application Description
Tested Reactivity Human
Immunogen BTC fusion protein Ag26724 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name betacellulin
Synonyms betacellulin, BTC, Probetacellulin
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC011618
Gene Symbol BTC
Gene ID (NCBI) 685
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP35070
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, binds and activates ErbB1 and ErbB4 homodimers. BTC is synthesized primarily as a transmembrane precursor, which is then processed to mature molecule by proteolytic events. This protein is a ligand for the EGF receptor. BTC was expressed in tumors and involved in tumor growth progression.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 BTC antibody CL488-66683Download protocol
Standard Protocols
Click here to view our Standard Protocols