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FKBP8 Monoclonal antibody

FKBP8 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1C1G12

Cat no : 66690-1-Ig

Print datasheet

Synonyms

38 kDa FK506 binding protein, 38 kDa FKBP, FK506 binding protein 8, FK506 binding protein 8, 38kDa, FKBP 38, FKBP 8, FKBP38, FKBP8, FKBPr38, hFKBP38, PPIase FKBP8, Rotamase

验证数据展示

  • WB analysis of Jurkat using 66690-1-Ig

    WB analysis of Jurkat using 66690-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66690-1-Ig

    WB analysis using 66690-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66690-1-Ig (FKBP8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 66690-1-Ig

    IF Staining of A431 using 66690-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FKBP8 antibody (66690-1-Ig, Clone: 1C1G12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IF/ICC detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

CoIPSee 1 publications below

产品信息

66690-1-Ig targets FKBP8 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsCoIP
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen FKBP8 fusion protein Ag1649 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name FK506 binding protein 8, 38kDa
Synonyms 38 kDa FK506 binding protein, 38 kDa FKBP, FK506 binding protein 8, FK506 binding protein 8, 38kDa, FKBP 38, FKBP 8, FKBP38, FKBP8, FKBPr38, hFKBP38, PPIase FKBP8, Rotamase
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC009966
Gene Symbol FKBP8
Gene ID (NCBI) 23770
RRIDAB_2882044
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

FK506 binding protein 8 (FKBP8), is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP8 does not seem to have PPIase/rotamase activity, which is different from other members of this family. The active form of FKBP8 may play a role in the regulation of apoptosis. FKBP8 has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for FKBP8 antibody 66690-1-IgDownload protocol
IF protocol for FKBP8 antibody 66690-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanCoIP

J Biol Chem

Lysine demethylase KDM1A promotes cell growth via FKBP8-BCL2 axis in hepatocellular carcinoma

Authors - Suli Lv
View Article