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ATP5O Monoclonal antibody

ATP5O Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D3B8

Cat no : 66696-1-Ig

Print datasheet

Synonyms

ATP5O, ATPO, OSCP

验证数据展示

  • IF Staining of HeLa using 66696-1-Ig

    IF Staining of HeLa using 66696-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66696-1-Ig (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 66696-1-Ig

    WB analysis using 66696-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66696-1-Ig (ATP5O antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 66696-1-Ig

    IHC staining of human liver cancer using 66696-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66696-1-Ig

    IHC staining of human liver cancer using 66696-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells, A549 cells, NCI-H1299 cells, Jurkat cells, K-562 cells, THP-1 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66696-1-Ig targets ATP5O in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen ATP5O fusion protein Ag1458 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Synonyms ATP5O, ATPO, OSCP
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC021233
Gene Symbol ATP5O
Gene ID (NCBI) 539
RRIDAB_2882049
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48047
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.

实验方案

Product Specific Protocols
WB protocol for ATP5O antibody 66696-1-IgDownload protocol
IHC protocol for ATP5O antibody 66696-1-IgDownload protocol
IF protocol for ATP5O antibody 66696-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Science

Mitochondrial remodeling and ischemic protection by G protein-coupled receptor 35 agonists

Authors - Gregory A Wyant
View Article