• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ATP5O Monoclonal antibody, PBS Only

ATP5O Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1D3B8

Cat No : 66696-1-PBS

Print datasheet

Synonyms

ATP5O, ATPO, OSCP

验证数据展示

  • IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

    IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66696-1-Ig (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • WB analysis using 66696-1-Ig (same clone as 66696-1-PBS)

    WB analysis using 66696-1-Ig (same clone as 66696-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66696-1-Ig (ATP5O antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

产品信息

66696-1-PBS targets ATP5O in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen ATP5O fusion protein Ag1458 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Synonyms ATP5O, ATPO, OSCP
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC021233
Gene Symbol ATP5O
Gene ID (NCBI) 539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48047
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.