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验证数据展示

  • IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

    IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66696-1-Ig (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • WB analysis using 66696-1-Ig (same clone as 66696-1-PBS)

    WB analysis using 66696-1-Ig (same clone as 66696-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66696-1-Ig (ATP5O antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

ATP5O Monoclonal antibody, PBS Only

ATP5O Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66696-1-PBS

产品说明书

CloneNo. 1D3B8

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ATP5O, ATPO, OSCP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66696-1-PBS targets ATP5O in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 ATP5O fusion protein Ag1458 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
别名 ATP5O, ATPO, OSCP
计算分子量 23 kDa
观测分子量 23-25 kDa
GenBank蛋白编号BC021233
基因名称 ATP5O
Gene ID (NCBI) 539
RRIDAB_2882049
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48047
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.