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ATP5O Monoclonal antibody, PBS Only

ATP5O Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D3B8

Cat no : 66696-1-PBS

Print datasheet

Synonyms

ATP5O, ATPO, OSCP

验证数据展示

  • IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

    IF Staining of HeLa using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66696-1-Ig (ATP5O antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • WB analysis using 66696-1-Ig (same clone as 66696-1-PBS)

    WB analysis using 66696-1-Ig (same clone as 66696-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66696-1-Ig (ATP5O antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    IHC staining of human liver cancer using 66696-1-Ig (same clone as 66696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66696-1-Ig (ATP5O antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66696-1-PBS in a different storage buffer formulation.

产品信息

66696-1-PBS targets ATP5O in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen ATP5O fusion protein Ag1458 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit
Synonyms ATP5O, ATPO, OSCP
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23-25 kDa
GenBank Accession NumberBC021233
Gene Symbol ATP5O
Gene ID (NCBI) 539
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48047
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.