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验证数据展示

  • WB analysis using 66752-1-Ig

    WB analysis using 66752-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66752-1-Ig

    IF Staining of HepG2 using 66752-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PLA2G4D antibody (66752-1-Ig, Clone: 2E2A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PLA2G4D Monoclonal antibody

PLA2G4D Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66752-1-Ig

产品说明书

CloneNo. 2E2A10

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, SMMC-7721 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66752-1-Ig targets PLA2G4D in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 PLA2G4D fusion protein Ag4178 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase A2, group IVD (cytosolic)
别名 cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D
计算分子量 818 aa, 92 kDa
观测分子量 92 kDa
GenBank蛋白编号BC034571
基因名称 PLA2G4D
Gene ID (NCBI) 283748
RRIDAB_2882098
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ86XP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).

实验方案

Product Specific Protocols
WB protocol for PLA2G4D antibody 66752-1-IgDownload protocol
IF protocol for PLA2G4D antibody 66752-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols