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验证数据展示

  • IF Staining of HepG2 using CL488-66752

    IF Staining of HepG2 using CL488-66752

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PLA2G4D antibody (CL488-66752, Clone: 2E2A10 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated PLA2G4D Monoclonal antibody

PLA2G4D Monoclonal Antibody for IF/ICC
Cat No. CL488-66752

Print datasheet

CloneNo. 2E2A10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66752-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66752 targets PLA2G4D in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen PLA2G4D fusion protein Ag4178 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase A2, group IVD (cytosolic)
Synonyms cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D
Calculated Molecular Weight 818 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC034571
Gene Symbol PLA2G4D
Gene ID (NCBI) 283748
RRIDAB_2934493
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86XP0
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PLA2G4D antibody CL488-66752Download protocol
Standard Protocols
Click here to view our Standard Protocols