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PLA2G4D Monoclonal antibody

PLA2G4D Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2E2A10

Cat no : 66752-1-Ig

Print datasheet

Synonyms

cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

验证数据展示

  • WB analysis using 66752-1-Ig

    WB analysis using 66752-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66752-1-Ig

    IF Staining of HepG2 using 66752-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PLA2G4D antibody (66752-1-Ig, Clone: 2E2A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, SMMC-7721 cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66752-1-Ig targets PLA2G4D in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen PLA2G4D fusion protein Ag4178 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase A2, group IVD (cytosolic)
Synonyms cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D
Calculated Molecular Weight 818 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC034571
Gene Symbol PLA2G4D
Gene ID (NCBI) 283748
RRIDAB_2882098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ86XP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).

实验方案

Product Specific Protocols
WB protocol for PLA2G4D antibody 66752-1-IgDownload protocol
IF protocol for PLA2G4D antibody 66752-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols