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KCNQ2 Monoclonal antibody

KCNQ2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1D4A1

Cat no : 66774-1-Ig

Print datasheet

Synonyms

HNSPC, ENB1, EBN1, EBN, BFNC

验证数据展示

  • WB analysis using 66774-1-Ig

    WB analysis using 66774-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66774-1-Ig (KCNQ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 66774-1-Ig

    IHC staining of mouse brain using 66774-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66774-1-Ig (KCNQ2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 66774-1-Ig

    IF Staining of U2OS using 66774-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using KCNQ2 antibody (66774-1-Ig, Clone: 1D4A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inpig brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66774-1-Ig targets KCNQ2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat, pig
Cited Reactivitymouse, rat
Immunogen KCNQ2 fusion protein Ag25781 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name potassium voltage-gated channel, KQT-like subfamily, member 2
Synonyms HNSPC, ENB1, EBN1, EBN, BFNC
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC000699
Gene Symbol KCNQ2
Gene ID (NCBI) 3785
RRIDAB_2882120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KCNQ2 antibody 66774-1-IgDownload protocol
IHC protocol for KCNQ2 antibody 66774-1-IgDownload protocol
IF protocol for KCNQ2 antibody 66774-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Rep

Mir324 knockout regulates the structure of dendritic spines and impairs hippocampal long-term potentiation

Authors - Emma V Parkins
View Article
mouseWB,IF

Hippocampus

Impacted spike frequency adaptation associated with reduction of KCNQ2/3 exacerbates seizure activity in temporal lobe epilepsy

Authors - Shicheng Jiang
View Article
ratWB

Clin Exp Hypertens

Effect of allisartan on blood pressure and left ventricular hypertrophy through Kv1.5 channels in hypertensive rats.

Authors - Chunfang Xu
View Article