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验证数据展示

  • WB analysis using 66774-1-Ig (same clone as 66774-1-PBS)

    WB analysis using 66774-1-Ig (same clone as 66774-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66774-1-Ig (KCNQ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66774-1-Ig (same clone as 66774-1-PBS)

    IHC staining of mouse brain using 66774-1-Ig (same clone as 66774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66774-1-Ig (KCNQ2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 66774-1-Ig (same clone as 66774-1-PBS)

    IF Staining of U2OS using 66774-1-Ig (same clone as 66774-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using KCNQ2 antibody (66774-1-Ig, Clone: 1D4A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50721-2

    Cytometric bead array standard curve of MP50721-2

    Cytometric bead array standard curve of MP50721-2, KCNQ2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66774-4-PBS. Detection antibody: 66774-1-PBS. Standard:Ag25781. Range: 0.781-100 ng/mL.

KCNQ2 Monoclonal antibody, PBS Only (Detector)

KCNQ2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66774-1-PBS

产品说明书

CloneNo. 1D4A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

HNSPC, ENB1, EBN1, EBN, BFNC

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66774-1-PBS targets KCNQ2 as part of a matched antibody pair:

MP50721-2: 66774-4-PBS capture and 66774-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 KCNQ2 fusion protein Ag25781 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 potassium voltage-gated channel, KQT-like subfamily, member 2
别名 HNSPC, ENB1, EBN1, EBN, BFNC
计算分子量 96 kDa
观测分子量 90 kDa
GenBank蛋白编号BC000699
基因名称 KCNQ2
Gene ID (NCBI) 3785
RRIDAB_2882120
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43526
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C