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KCNQ2 Monoclonal antibody, PBS Only (Detector)

KCNQ2 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D4A1

Cat no : 66774-1-PBS

Print datasheet

Synonyms

HNSPC, ENB1, EBN1, EBN, BFNC

验证数据展示

  • WB analysis using 66774-1-Ig (same clone as 66774-1-PBS)

    WB analysis using 66774-1-Ig (same clone as 66774-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66774-1-Ig (KCNQ2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 66774-1-Ig (same clone as 66774-1-PBS)

    IHC staining of mouse brain using 66774-1-Ig (same clone as 66774-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66774-1-Ig (KCNQ2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 66774-1-Ig (same clone as 66774-1-PBS)

    IF Staining of U2OS using 66774-1-Ig (same clone as 66774-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using KCNQ2 antibody (66774-1-Ig, Clone: 1D4A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66774-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50721-2

    Cytometric bead array standard curve of MP50721-2

    Cytometric bead array standard curve of MP50721-2, KCNQ2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66774-4-PBS. Detection antibody: 66774-1-PBS. Standard:Ag25781. Range: 0.781-100 ng/mL.

产品信息

66774-1-PBS targets KCNQ2 as part of a matched antibody pair:

MP50721-2: 66774-4-PBS capture and 66774-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen KCNQ2 fusion protein Ag25781 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name potassium voltage-gated channel, KQT-like subfamily, member 2
Synonyms HNSPC, ENB1, EBN1, EBN, BFNC
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC000699
Gene Symbol KCNQ2
Gene ID (NCBI) 3785
RRIDAB_2882120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43526
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C