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验证数据展示

  • WB analysis of pig cartilage using 66793-1-Ig

    WB analysis of pig cartilage using 66793-1-Ig

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66793-1-Ig (COMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66793-1-Ig

    IF Staining of HepG2 using 66793-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of U2OS using 66793-1-Ig

    IF Staining of U2OS using 66793-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

COMP Monoclonal antibody

COMP Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66793-1-Ig

产品说明书

CloneNo. 1D7A6

宿主/亚型

Mouse / IgG1

种属反应性

Human, pig

应用

WB, IF/ICC, ELISA

别名

COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig cartilage tissue
Positive IF/ICC detected inHepG2 cells, U2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66793-1-Ig targets COMP in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, pig
免疫原 COMP fusion protein Ag4575 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cartilage oligomeric matrix protein
别名 COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5
计算分子量 757 aa, 83 kDa
观测分子量 100-130 kDa
GenBank蛋白编号BC033676
基因名称 COMP
Gene ID (NCBI) 1311
RRIDAB_2882137
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP49747
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others. This antibody got 100-130 kDa maybe due to glycosylation.

实验方案

Product Specific Protocols
WB protocol for COMP antibody 66793-1-IgDownload protocol
IF protocol for COMP antibody 66793-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols