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COMP Monoclonal antibody

COMP Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D7A6

Cat No : 66793-1-Ig

Print datasheet

Synonyms

COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5

验证数据展示

  • WB analysis of pig cartilage using 66793-1-Ig

    WB analysis of pig cartilage using 66793-1-Ig

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66793-1-Ig (COMP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 66793-1-Ig

    IF Staining of HepG2 using 66793-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of U2OS using 66793-1-Ig

    IF Staining of U2OS using 66793-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using COMP antibody (66793-1-Ig, Clone: 1D7A6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig cartilage tissue
Positive IF detected inHepG2 cells, U2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66793-1-Ig targets COMP in WB, IF, ELISA applications and shows reactivity with Human, pig samples.

Tested Applications IF/ICC, WB, ELISA Application Description
Tested Reactivity Human, pig
Immunogen COMP fusion protein Ag4575 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cartilage oligomeric matrix protein
Synonyms COMP, EDM1, EPD1, MED, PSACH, THBS5, Thrombospondin 5, TSP5
Calculated Molecular Weight 757 aa, 83 kDa
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberBC033676
Gene Symbol COMP
Gene ID (NCBI) 1311
RRIDAB_2882137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COMP is a secreted pentameric glycoprotein built from modular units and belongs to the thrombospondin protein family. COMP is expressed by hypertrophic chondrocytes and osteoblasts during endochondral ossification, and its expression is increased during fracture healing. COMP is involved in the assembly of collagen fibrils and other structural matrix components. COMP interacts with a number of cartilage ECM proteins, including fibronectin, collagen I, II IX, XII, and XIV, matrilins, as well as proteoglycans such as aggrecan and others. This antibody got 100-130 kDa maybe due to glycosylation.

实验方案

Product Specific Protocols
WB protocol for COMP antibody 66793-1-IgDownload protocol
IF protocol for COMP antibody 66793-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols