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验证数据展示

  • WB analysis of U-937 using 66812-1-Ig

    WB analysis of U-937 using 66812-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 66812-1-Ig

    IF Staining of A431 using 66812-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cystatin B antibody (66812-1-Ig, Clone: 2G9B8 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human skin cancer using 66812-1-Ig

    IHC staining of human skin cancer using 66812-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66812-1-Ig (Cystatin B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSTB antibody included
  • IHC staining of human skin cancer using 66812-1-Ig

    IHC staining of human skin cancer using 66812-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66812-1-Ig (Cystatin B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSTB antibody included

Cystatin B Monoclonal antibody

Cystatin B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66812-1-Ig

产品说明书

CloneNo. 2G9B8

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CSTB, Cystatin-B, CST6, CPI-B, CPI B

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells, human saliva tissue, THP-1 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CSTB Ready-To-Use IHC Kit. CSTB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66812-1-Ig targets Cystatin B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Cystatin B fusion protein Ag21291 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cystatin B (stefin B)
别名 CSTB, Cystatin-B, CST6, CPI-B, CPI B
计算分子量 11 kDa
观测分子量 11-14 kDa
GenBank蛋白编号BC003370
基因名称 Cystatin B
Gene ID (NCBI) 1476
RRIDAB_2882155
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04080
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.

实验方案

Product Specific Protocols
WB protocol for Cystatin B antibody 66812-1-IgDownload protocol
IHC protocol for Cystatin B antibody 66812-1-IgDownload protocol
IF protocol for Cystatin B antibody 66812-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols