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Cystatin B Monoclonal antibody, PBS Only

Cystatin B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G9B8

Cat no : 66812-1-PBS

Print datasheet

Synonyms

CSTB, Cystatin-B, CST6, CPI-B, CPI B

验证数据展示

  • WB analysis of U-937 using 66812-1-Ig (same clone as 66812-1-PBS)

    WB analysis of U-937 using 66812-1-Ig (same clone as 66812-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 66812-1-Ig (Cystatin B antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66812-1-Ig (same clone as 66812-1-PBS)

    IF Staining of A431 using 66812-1-Ig (same clone as 66812-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using Cystatin B antibody (66812-1-Ig, Clone: 2G9B8 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

  • IHC staining of human skin cancer using 66812-1-Ig (same clone as 66812-1-PBS)

    IHC staining of human skin cancer using 66812-1-Ig (same clone as 66812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66812-1-Ig (Cystatin B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSTB antibody included
  • IHC staining of human skin cancer using 66812-1-Ig (same clone as 66812-1-PBS)

    IHC staining of human skin cancer using 66812-1-Ig (same clone as 66812-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66812-1-Ig (Cystatin B antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66812-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSTB antibody included
Planning an IHC experiment? We recommend our IHCeasy CSTB Ready-To-Use IHC Kit. CSTB primary antibody included.

产品信息

66812-1-PBS targets Cystatin B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Cystatin B fusion protein Ag21291 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cystatin B (stefin B)
Synonyms CSTB, Cystatin-B, CST6, CPI-B, CPI B
Calculated Molecular Weight 11 kDa
Observed Molecular Weight 11-14 kDa
GenBank Accession NumberBC003370
Gene Symbol Cystatin B
Gene ID (NCBI) 1476
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP04080
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cystatin B (CSTB), a member of the cystatin superfamily protein, is a stefin that functions as an intracellular thiol protease inhibitor and has been thought to play a role in protecting against the proteases leaking from lysosomes. CSTB plays various functions in a variety of diseases, including epithelial ovarian cancer, colon cancer, and myoclonus epilepsy. Evidence indicates that mutations in CSTB are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies.