验证数据展示
经过测试的应用
| Positive WB detected in | T-47D cells, LNCaP cells, MCF-7 cells |
| Positive IHC detected in | human thyroid cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DIO2 Ready-To-Use IHC Kit. DIO2 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66813-1-Ig targets DIO2 in WB, IHC, RIP, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, RIP |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | DIO2 fusion protein Ag24057 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | deiodinase, iodothyronine, type II |
| 别名 | 5DII, D2, DIO2, DIOII, ITDI2, SelY, TXDI2, Type 2 DI, Type II 5 deiodinase |
| 计算分子量 | 273 aa, 31 kDa |
| 观测分子量 | 30-35 kDa |
| GenBank蛋白编号 | BC074882 |
| 基因名称 | DIO2 |
| Gene ID (NCBI) | 1734 |
| RRID | AB_2882156 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q92813 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DIO2 antibody 66813-1-Ig | Download protocol |
| IHC protocol for DIO2 antibody 66813-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy Defective autophagy contributes to endometrial epithelial-mesenchymal transition in intrauterine adhesions.
| ||
Biochem Biophys Rep A prediction model for prognosis of gastric adenocarcinoma based on six metabolism-related genes | ||
Biochem Pharmacol Dysregulated lncSNHG12 suppresses the invasion and migration of trophoblasts by regulating Dio2/Snail axis to involve in recurrent spontaneous abortion
| ||
Thyroid Increased thyroid hormone action alleviates hippocampal damage by down-regulating neuronal type-Ⅰ interferon signaling/necroptosis in diabetes-associated cognitive dysfunction |
