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验证数据展示

  • WB analysis of T-47D using 66813-1-Ig (same clone as 66813-1-PBS)

    WB analysis of T-47D using 66813-1-Ig (same clone as 66813-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66813-1-Ig (same clone as 66813-1-PBS)

    WB analysis of MCF-7 using 66813-1-Ig (same clone as 66813-1-PBS)

    WB result of DIO2 antibody (66813-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIO2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DIO2 antibody included
  • IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DIO2 antibody included
  • Featured Product
  • KD/KO验证

DIO2 Monoclonal antibody, PBS Only

DIO2 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66813-1-PBS

产品说明书

CloneNo. 1F2A1

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, ELISA

别名

5DII, D2, DIO2, DIOII, ITDI2, SelY, TXDI2, Type 2 DI, Type II 5 deiodinase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DIO2 Ready-To-Use IHC Kit. DIO2 primary antibody included.

产品信息

66813-1-PBS targets DIO2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 DIO2 fusion protein Ag24057 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 deiodinase, iodothyronine, type II
别名 5DII, D2, DIO2, DIOII, ITDI2, SelY, TXDI2, Type 2 DI, Type II 5 deiodinase
计算分子量 273 aa, 31 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC074882
基因名称 DIO2
Gene ID (NCBI) 1734
RRIDAB_2882156
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92813
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.