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  • Featured Product
  • KD/KO Validated

DIO2 Monoclonal antibody, PBS Only

DIO2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1F2A1

Cat no : 66813-1-PBS

Print datasheet

Synonyms

5DII, D2, DIO2, DIOII, ITDI2, SelY, TXDI2, Type 2 DI, Type II 5 deiodinase

验证数据展示

  • WB analysis of T-47D using 66813-1-Ig (same clone as 66813-1-PBS)

    WB analysis of T-47D using 66813-1-Ig (same clone as 66813-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66813-1-Ig (same clone as 66813-1-PBS)

    WB analysis of MCF-7 using 66813-1-Ig (same clone as 66813-1-PBS)

    WB result of DIO2 antibody (66813-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIO2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

  • IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DIO2 antibody included
  • IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DIO2 antibody included
Planning an IHC experiment? We recommend our IHCeasy DIO2 Ready-To-Use IHC Kit. DIO2 primary antibody included.

产品信息

66813-1-PBS targets DIO2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen DIO2 fusion protein Ag24057 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name deiodinase, iodothyronine, type II
Synonyms 5DII, D2, DIO2, DIOII, ITDI2, SelY, TXDI2, Type 2 DI, Type II 5 deiodinase
Calculated Molecular Weight 273 aa, 31 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC074882
Gene Symbol DIO2
Gene ID (NCBI) 1734
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92813
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.