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CHAT Monoclonal antibody

CHAT Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F7A9

Cat no : 66816-1-Ig

Print datasheet

Synonyms

CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2

验证数据展示

  • WB analysis using 66816-1-Ig

    WB analysis using 66816-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, PC-12 cells, HEK-293 cells, SH-SY5Y cells, Y79 cells, U-251 cells, Neuro-2a cells
Planning an IHC experiment? We recommend our IHCeasy CHAT Ready-To-Use IHC Kit. CHAT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66816-1-Ig targets CHAT in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CHAT fusion protein Ag19602 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name choline acetyltransferase
Synonyms CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2
Calculated Molecular Weight 748 aa, 83 kDa
Observed Molecular Weight 67-71 kDa
GenBank Accession NumberBC130617
Gene Symbol CHAT
Gene ID (NCBI) 1103
RRIDAB_2882159
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28329
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.

实验方案

Product Specific Protocols
WB protocol for CHAT antibody 66816-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols