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验证数据展示

  • WB analysis using 66816-1-Ig

    WB analysis using 66816-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 66816-1-Ig

    IHC staining of mouse small intestine using 66816-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHAT antibody included
  • IHC staining of mouse small intestine using 66816-1-Ig

    IHC staining of mouse small intestine using 66816-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHAT antibody included
  • IHC staining of rat small intestine using 66816-1-Ig

    IHC staining of rat small intestine using 66816-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CHAT antibody included

CHAT Monoclonal antibody

CHAT Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66816-1-Ig

产品说明书

CloneNo. 1F7A9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EC:2.3.1.6, Choline O-acetyltransferase, choline acetyltransferase, Choline acetylase, CHOACTase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, PC-12 cells, HEK-293 cells, SH-SY5Y cells, Y79 cells, U-251 cells, Neuro-2a cells
Positive IHC detected inmouse small intestine tissue, rat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CHAT Ready-To-Use IHC Kit. CHAT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66816-1-Ig targets CHAT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHAT fusion protein Ag19602 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 choline acetyltransferase
别名 EC:2.3.1.6, Choline O-acetyltransferase, choline acetyltransferase, Choline acetylase, CHOACTase
计算分子量 748 aa, 83 kDa
观测分子量 67-71 kDa
GenBank蛋白编号BC130617
基因名称 CHAT
Gene ID (NCBI) 1103
RRIDAB_2882159
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28329
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.

实验方案

Product Specific Protocols
WB protocol for CHAT antibody 66816-1-IgDownload protocol
IHC protocol for CHAT antibody 66816-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols