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验证数据展示

  • WB analysis using 66816-1-Ig (same clone as 66816-1-PBS)

    WB analysis using 66816-1-Ig (same clone as 66816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHAT antibody included
  • IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHAT antibody included
  • IHC staining of rat small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    IHC staining of rat small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHAT antibody included

CHAT Monoclonal antibody, PBS Only

CHAT Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66816-1-PBS

产品说明书

CloneNo. 1F7A9

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

EC:2.3.1.6, Choline O-acetyltransferase, choline acetyltransferase, Choline acetylase, CHOACTase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CHAT Ready-To-Use IHC Kit. CHAT primary antibody included.

产品信息

66816-1-PBS targets CHAT in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CHAT fusion protein Ag19602 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 choline acetyltransferase
别名 EC:2.3.1.6, Choline O-acetyltransferase, choline acetyltransferase, Choline acetylase, CHOACTase
计算分子量 748 aa, 83 kDa
观测分子量 67-71 kDa
GenBank蛋白编号BC130617
基因名称 CHAT
Gene ID (NCBI) 1103
RRIDAB_2882159
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP28329
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.