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CHAT Monoclonal antibody, PBS Only

CHAT Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F7A9

Cat no : 66816-1-PBS

Print datasheet

Synonyms

CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2

验证数据展示

  • WB analysis using 66816-1-Ig (same clone as 66816-1-PBS)

    WB analysis using 66816-1-Ig (same clone as 66816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CHAT Ready-To-Use IHC Kit. CHAT primary antibody included.

产品信息

66816-1-PBS targets CHAT in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CHAT fusion protein Ag19602 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name choline acetyltransferase
Synonyms CHAT, CHOACTase, Choline acetylase, choline acetyltransferase, Choline O acetyltransferase, CMS1A, CMS1A2
Calculated Molecular Weight 748 aa, 83 kDa
Observed Molecular Weight 67-71 kDa
GenBank Accession NumberBC130617
Gene Symbol CHAT
Gene ID (NCBI) 1103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28329
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.