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ACVRL1 Monoclonal antibody

ACVRL1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E11B10

Cat no : 66857-1-Ig

Print datasheet

Synonyms

Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I

验证数据展示

  • WB analysis of HUVEC using 66857-1-Ig

    WB analysis of HUVEC using 66857-1-Ig

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66857-1-Ig (ACVRL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 66857-1-Ig

    IHC staining of human lymphoma using 66857-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66857-1-Ig (ACVRL1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 66857-1-Ig

    IHC staining of human lymphoma using 66857-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 66857-1-Ig (ACVRL1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHUVEC cells, Daudi cells, human placenta tissue, JAR cells, Jurkat cells, mouse brain tissue
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66857-1-Ig targets ACVRL1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen ACVRL1 fusion protein Ag6446 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name activin A receptor type II-like 1
Synonyms Activin receptor like kinase 1, ACVRL1, ACVRLK1, ALK 1, ALK1, HHT, HHT2, ORW2, SKR3, TSR I
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 56 kDa, 63 kDa
GenBank Accession NumberBC042637
Gene Symbol ACVRL1
Gene ID (NCBI) 94
RRIDAB_2882196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP37023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACVRL1 (also known as ALK1) is a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. ACVRL1 is highly expressed in endothelial cells and has a critical role in the control of blood vessel development and repair (PMID: 8640225). Mutations in the ACVRL1 gene are associated with hemorrhagic telangiectasia type 2.

实验方案

Product Specific Protocols
WB protocol for ACVRL1 antibody 66857-1-IgDownload protocol
IHC protocol for ACVRL1 antibody 66857-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols