SPON2 Monoclonal antibody
SPON2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1H4B11
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
66859-1-Ig targets SPON2 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | SPON2 fusion protein Ag14337 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | spondin 2, extracellular matrix protein |
| Synonyms | DIL 1, DIL1, M spondin, Mindin, SPON2, Spondin 2 |
| Calculated Molecular Weight | 331 aa, 36 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC002707 |
| Gene Symbol | SPON2 |
| Gene ID (NCBI) | 10417 |
| RRID | AB_2882198 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BUD6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SPON2 (Spondin 2), also known as Mindin, is a member of the F-spondin family of secreted extracellular matrix proteins. This protein has been implicated in axon guidance, immune response and adhesion. SPON2 is essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (PMID: 14691481). It also functions as an integrin ligand for inflammatory cell recruitment and T-cell priming (PMID: 19153605). Besides, SPON2 has been reported as a prognostic biomarker for ovarian and prostate cancer (PMID: 17490732; 22615945; 23665271).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SPON2 antibody 66859-1-Ig | Download protocol |
| IHC protocol for SPON2 antibody 66859-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |