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KPNA2 Monoclonal antibody

KPNA2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G9C1

Cat no : 66870-1-Ig

Print datasheet

Synonyms

Karyopherin subunit alpha-2, IPOA1, IPOA 1, IPO A1, Importin subunit alpha-1

验证数据展示

  • WB analysis using 66870-1-Ig

    WB analysis using 66870-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66870-1-Ig (KPNA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 66870-1-Ig

    IHC staining of human skin cancer using 66870-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 66870-1-Ig (KPNA2 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KPNA2 antibody included
  • IF Staining of MCF-7 using 66870-1-Ig

    IF Staining of MCF-7 using 66870-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KPNA2 antibody (66870-1-Ig, Clone: 2G9C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, Raji cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KPNA2 Ready-To-Use IHC Kit. KPNA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66870-1-Ig targets KPNA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen KPNA2 fusion protein Ag28386 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Synonyms Karyopherin subunit alpha-2, IPOA1, IPOA 1, IPO A1, Importin subunit alpha-1
Calculated Molecular Weight 57.8 kDa
Observed Molecular Weight 55-58 kDa
GenBank Accession NumberBC005978
Gene Symbol KPNA2
Gene ID (NCBI) 3838
RRIDAB_2882207
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP52292
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KPNA2 (karyopherin α2) is a nuclear import factor involved in the nucleocytoplasmic transport system. It is functionally involved in the tumor progression and is elevated in multiple cancers. KPNA2 expression may be a useful prognostic biomarker to monitor cancer prognosis.

实验方案

Product Specific Protocols
WB protocol for KPNA2 antibody 66870-1-IgDownload protocol
IHC protocol for KPNA2 antibody 66870-1-IgDownload protocol
IF protocol for KPNA2 antibody 66870-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols