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验证数据展示

  • WB analysis using 66899-1-Ig (same clone as 66899-1-PBS)

    WB analysis using 66899-1-Ig (same clone as 66899-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66899-1-Ig (SPTLC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

    IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

    IHC staining of human colon cancer using 66899-1-Ig (same clone as 66899-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66899-1-Ig (SPTLC1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66899-1-Ig (same clone as 66899-1-PBS)

    WB analysis of HEK-293 using 66899-1-Ig (same clone as 66899-1-PBS)

    WB result of SPTLC1 antibody (66899-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SPTLC1 transfected HEK-293 cells. This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66899-1-Ig (same clone as 66899-1-PBS)

    IF Staining of HepG2 using 66899-1-Ig (same clone as 66899-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SPTLC1 antibody (66899-1-Ig, Clone: 2D11D7 ) at dilution of 1:981 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66899-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50865-2

    Cytometric bead array standard curve of MP50865-2

    Cytometric bead array standard curve of MP50865-2, SPTLC1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66899-4-PBS. Detection antibody: 66899-1-PBS. Standard:Ag7876. Range: 0.195-100 ng/mL.

  • Featured Product
  • KD/KO验证

SPTLC1 Monoclonal antibody, PBS Only (Detector)

SPTLC1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 66899-1-PBS

产品说明书

CloneNo. 2D11D7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66899-1-PBS targets SPTLC1 as part of a matched antibody pair:

MP50865-2: 66899-4-PBS capture and 66899-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SPTLC1 fusion protein Ag7876 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 serine palmitoyltransferase, long chain base subunit 1
别名 Serine-palmitoyl-CoA transferase 1, LCB1, LCB 1, HSAN1, EC:2.3.1.50
计算分子量 53 kDa
观测分子量 55 kDa
GenBank蛋白编号BC007085
基因名称 SPTLC1
Gene ID (NCBI) 10558
RRIDAB_2882228
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO15269
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SPTLC1 is a subunit of serine palmitoyltransferase (SPT) which is the key enzyme in sphingolipid biosynthesis and is essential for embryogenesis and cell survival. Mutations in the SPTLC1 gene (C133W, C133Y, V144D, and G387A) were reported to be responsible for the development of an inherited sensory neuropathy (hereditary sensory neuropathy type I, HSN1).